➦   Lipid production in Yarrowia lipolytica is maximized by engineering cytosolic redox metabolism

➦   Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding trans

➦   Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine de

➦   Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells

➦   Genome-wide target specificities of CRISPR RNA-guided programmable deaminases

➦   Transplantation of engineered organoids enables rapid generation of metastatic mouse models of color

➦   In vivo genome editing and organoid transplantation models of colorectal cancer and metastasis

➦   Digital-to-biological converter for on-demand production of biologics

➦   Rapid cloning of genes in hexaploid wheat using cultivar-specific long-range chromosome assembly

➦   Guided self-organization and cortical plate formation in human brain organoids

➦   Engineered bacteria can function in the mammalian gut long-term as live diagnostics of inflammation

➦   Computational design of trimeric influenza-neutralizing proteins targeting the hemagglutinin recepto

➦   Engineered Cpf1 variants with altered PAM specificities

➦   A calcium- and light-gated switch to induce gene expression in activated neurons

➦   A light- and calcium-gated transcription factor for imaging and manipulating activated neurons

➦   Detection of dysregulated protein-association networks by high-throughput proteomics predicts cancer

➦   Haplotype phasing of whole human genomes using bead-based barcode partitioning in a single tube

➦   Cell-type-specific metabolic labeling of nascent proteomes in vivo

➦   Single-cell deep phenotyping of IgG-secreting cells for high-resolution immune monitoring

➦   Retrieval of a million high-quality, full-length microbial 16S and 18S rRNA gene sequences without p

➦   Inhibition of 53BP1 favors homology-dependent DNA repair and increases CRISPR–Cas9 genome-editing ef

➦   A DNA nanorobot functions as a cancer therapeutic in response to a molecular trigger in vivo

➦   A highly specific SpCas9 variant is identified by in vivo screening in yeast

➦   Engineered promoters enable constant gene expression at any copy number in bacteria

➦   IL-7 and CCL19 expression in CAR-T cells improves immune cell infiltration and CAR-T cell survival i

➦   Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy

➦   Simultaneous lineage tracing and cell-type identification using CRISPR–Cas9-induced genetic scars

➦   High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR–Cas

➦   Secure genome-wide association analysis using multiparty computation

➦   Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryo

➦   Photosynthetic artificial organelles sustain and control ATP-dependent reactions in a protocellular

➦   De novo DNA synthesis using polymerase-nucleotide conjugates

➦   Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylatio

➦   Reversal of indoleamine 2,3-dioxygenase–mediated cancer immune suppression by systemic kynurenine de

➦   Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrange

➦   Conversion of staphylococcal pathogenicity islands to CRISPR-carrying antibacterial agents that cure

➦   An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities

➦   Efficient proximity labeling in living cells and organisms with TurboID

➦   A universal SNP and small-indel variant caller using deep neural networks

➦   Genome editing of upstream open reading frames enables translational control in plants

➦   Optimized base editors enable efficient editing in cells, organoids and mice

➦   On-demand manufacturing of clinical-quality biopharmaceuticals

➦   Variation graph toolkit improves read mapping by representing genetic variation in the reference

➦   Revealing the cellular degradome by mass spectrometry analysis of proteasome-cleaved peptides

➦   Single-cell isoform RNA sequencing characterizes isoforms in thousands of cerebellar cells

➦   ClampFISH detects individual nucleic acid molecules using click chemistry–based amplification

➦   Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice

➦   T cell receptor fingerprinting enables in-depth characterization of the interactions governing recog

➦   A massively parallel reporter assay dissects the influence of chromatin structure on cis-regulatory

➦   Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes