DNA–based immunization by in vivo transfection of dendritic cells
Author: ["Cal Condon","Simon C. Watkins","Christina M. Celluzzi","Kathleen Thompson","Louis D. Falo Jr."]
Publication: Nature Medicine
CITE.CC academic search helps you expand the influence of your papers.
Abstract
Delivery of antigen in a manner that induces effective, antigen–specific immunity is a critical challenge in vaccine design. Optimal antigen presentation is mediated by professional antigen–presenting cells (APCs) capable of taking up, processing and presenting antigen to T cells in the context of costimulatory signals required for T–cell activation. Developing immunization strategies to optimize antigen presentation by dendritic cells, the most potent APCs, is a rational approach to vaccine design. Here we show that cutaneous genetic immunization with naked DNA results in potent, antigen–specific, cytotoxic T lymphocyte–mediated protective tumor immunity. This method of immunization results in the transfection of skin–derived dendritic cells, which localize in the draining lymph nodes. These observations provide a basis for further development of DNA–based vaccines and demonstrate the feasibility of genetically engineering dendritic cells in vivo.
Cite this article
Condon, C., Watkins, S., Celluzzi, C. et al. DNA–based immunization by in vivo transfection of dendritic cells. Nat Med 2, 1122–1128 (1996). https://doi.org/10.1038/nm1096-1122
