CITE.CC academic search helps you expand the influence of your papers.
Abstract
Human Parvovirus (B19), a small single stranded DNA virus, has been associated with a variety of different human diseases including aplastic crisis and erythema infectiosum. To date there is no practical source of viral antigen for screening potentially infected patients. Starting with previously isolated genomic clones and using a combination of in vitro and in vivo techniques, the major coding region of the B19 virion protein structural gene was expressed in E. coli as a 196 kD β-galactosidase fusion protein. Using this recombinant antigen, antibodies to B19 from the sera of ten patients known to be infected with the virus were detected by Western blot analysis. The sera from 50 normal healthy individuals were also tested and 44% of the subjects showed a positive immunoreaction to the B19-β-galactosidase fusion protein.Cite this article
Sisk, W., Berman, M. Expression of Human Parvovirus B19 Structural Protein in E. coli and Detection of Antiviral Antibodies in Human Serum. Nat Biotechnol 5, 1077–1080 (1987). https://doi.org/10.1038/nbt1087-1077 