Continuous Production of a Novel Lysozyme via Secretion from the Yeast, Pichia pastoris
Author: ["M. E. Digan","S. V. Lair","R. A. Brierley","R. S. Siegel","M. E. Williams","S. B. Ellis","P. A. Kellaris","S. A. Provow","W. S. Craig","G. Veliçelebi","M. M. Harpold","G. P. Thill"]
Publication: Bio/Technology
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Abstract
We have cloned a cDNA for bovine lysozyme c2, a novel lysozyme characteristic of the cow stomach and expressed its protein product by secretion using its native signal sequence in the methylotrophic yeast, Pichia pastoris. A semi–continuous fermentation process used for production resulted in extremely high cell densities and product concentrations. During fed–batch fermentation, bovine lysozyme concentrations exceeded 550 mg/liter, and cell densities exceeded 120 g dry cells/liter. In continuous culture, bovine lysozyme and cell concentrations remained constant at approximately 350 mg/liter and 100 g dry cells/liter, respectively. The recombinant product is a single biologically active species that constitutes >80% of the protein in media from expressing cells; the signal sequence of the pre–lysozyme is accurately processed.
Cite this article
Digan, M., Lair, S., Brierley, R. et al. Continuous Production of a Novel Lysozyme via Secretion from the Yeast, Pichia pastoris. Nat Biotechnol 7, 160–164 (1989). https://doi.org/10.1038/nbt0289-160
