Mitigation of nitrous oxide emissions from soils by Bradyrhizobium japonicum inoculation
Author: ["Manabu Itakura","Yoshitaka Uchida","Hiroko Akiyama","Yuko Takada Hoshino","Yumi Shimomura","Sho Morimoto","Kanako Tago","Yong Wang","Chihiro Hayakawa","Yusuke Uetake","Cristina Sánchez","Shima Eda","Masahito Hayatsu","Kiwamu Minamisawa"]
Publication: Nature Climate Change
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Abstract
Soybean hosts the symbiotic nitrogen-fixing soil bacterium Bradyrhizobium japonicum, that can produce the greenhouse gas nitrous oxide. This study shows that nitrous oxide emissions from soybean ecosystems can be biologically mitigated at a field scale by inoculation with strains of B. japonicum that have increased nitrous oxide reductase activity. Nitrous oxide (N2O) is a greenhouse gas that is also capable of destroying the ozone layer1. Agricultural soil is the largest source of N2O (ref. 2). Soybean is a globally important leguminous crop, and hosts symbiotic nitrogen-fixing soil bacteria (rhizobia) that can also produce N2O (ref. 3). In agricultural soil, N2O is emitted from fertilizer and soil nitrogen. In soybean ecosystems, N2O is also emitted from the degradation of the root nodules4. Organic nitrogen inside the nodules is mineralized to NH4+, followed by nitrification and denitrification that produce N2O. N2O is then emitted into the atmosphere or is further reduced to N2 by N2O reductase (N2OR), which is encoded by the nosZ gene. Pure culture and vermiculite pot experiments showed lower N2O emission by nosZ+ strains5 and nosZ++ strains (mutants with increased N2OR activity)6 of Bradyrhizobium japonicum than by nosZ− strains. A pot experiment using soil confirmed these results7. Although enhancing N2OR activity has been suggested as a N2O mitigation option8,9, this has never been tested in the field. Here, we show that post-harvest N2O emission from soybean ecosystems due to degradation of nodules can be mitigated by inoculation of nosZ+ and non-genetically modified organism nosZ++ strains of B. japonicum at a field scale.
Cite this article
Itakura, M., Uchida, Y., Akiyama, H. et al. Mitigation of nitrous oxide emissions from soils by Bradyrhizobium japonicum inoculation. Nature Clim Change 3, 208–212 (2013). https://doi.org/10.1038/nclimate1734
