Gradient-driven motion of multivalent ligand molecules along a surface functionalized with multiple

Abstract

The kinetics of multivalent (multisite) interactions at interfaces is poorly understood, despite its fundamental importance for molecular or biomolecular motion and molecular recognition events at biological interfaces. Here, we use fluorescence microscopy to monitor the spreading of mono-, di- and trivalent ligand molecules on a receptor-functionalized surface, and perform multiscale computer simulations to understand the surface diffusion mechanisms. Analogous to chemotaxis, we found that the spreading is directional (along a developing gradient of vacant receptor sites) and is strongly dependent on ligand valency and concentration of a competing monovalent receptor in solution. We identify multiple surface diffusion mechanisms, which we call walking, hopping and flying. The study shows that the interfacial behaviour of multivalent systems is much more complex than that of monovalent ones. The kinetics of multivalent interactions at interfaces is poorly understood despite its fundamental importance for (bio)molecular motion and molecular recognition events at biological interfaces. Here, directional spreading of multivalent molecules has been observed, and multiple surface diffusion mechanisms have been identified and dubbed walking, hopping and flying.

Cite this article

Perl, A., Gomez-Casado, A., Thompson, D. et al. Gradient-driven motion of multivalent ligand molecules along a surface functionalized with multiple receptors. Nature Chem 3, 317–322 (2011). https://doi.org/10.1038/nchem.1005

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