Controlled release of antibodies for long-term topical passive immunoprotection of female mice again
Author: ["Jill K. Sherwood","Larry Zeitlin","Kevin J. Whaley","Richard A. Cone","Mark Saltzman"]
Publication: Nature Biotechnology
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Abstract
Current methods for sexually transmitted diseases (STD) prophylaxis, which can be disruptive and inconvenient, must be used before each act of sexual intercourse, so a method that provides protection over the course of many acts is desirable. We used a mouse model of vaginally-transmitted herpes simplex virus 2 (HSV-2) infection to test polymeric controlled-release devices for sustained passive immunoprotection. Vaginal disks were prepared by dispersing a monoclonal antibody to HSV-2 (111-174) within a matrix of poly(ethylene-co-vinyl acetate); these disks released 2 to 40 μg/day of antibody into buffered water. When disks were placed in the vagina, large amounts of 111-174 (5 to 3,000 ng) were recovered from the vaginal fluid over the next 8 days. Mice were vaginally challenged with 10 ID50 of HSV-2 either 3 or 7 days after disk placement; no mice receiving 111-174 disks became infected, while 65% of control mice receiving identical disks with nonspecific IgG did. Controlled-release disks with 111-174 provided significant protection against HSV-2 infection (p < 0.005). This new technology for long-term STD prophylaxis should increase user compliance, a factor limiting the efficacy of current methods.
Cite this article
Sherwood, J., Zeitlin, L., Whaley, K. et al. Controlled release of antibodies for long-term topical passive immunoprotection of female mice against genital herpes. Nat Biotechnol 14, 468–471 (1996). https://doi.org/10.1038/nbt0496-468