Rab5 regulates motility of early endosomes on microtubules
Author: ["Erik Nielsen","Fedor Severin","Jonathan M. Backer","Anthony A. Hyman","Marino Zerial"]
Publication: Nature Cell Biology
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Abstract
The small GTPase Rab5 regulates membrane docking and fusion in the early endocytic pathway. Here we reveal a new role for Rab5 in the regulation of endosome interactions with the microtubule network. Using Rab5 fused to green fluorescent protein we show that Rab5-positive endosomes move on microtubules in vivo. In vitro, Rab5 stimulates both association of early endosomes with microtubules and early-endosome motility towards the minus ends of microtubules. Moreover, similarly to endosome membrane docking and fusion, Rab5-dependent endosome movement depends on the phosphatidylinositol-3-OH kinase hVPS34. Thus, Rab5 functionally links regulation of membrane transport, motility and intracellular distribution of early endosomes.
Cite this article
Nielsen, E., Severin, F., Backer, J. et al. Rab5 regulates motility of early endosomes on microtubules. Nat Cell Biol 1, 376–382 (1999). https://doi.org/10.1038/14075
