Cargo binding and regulatory sites in the tail of fungal conventional kinesin

Abstract

Here, using a quantitative in vivo assay, we map three regions in the carboxy terminus of conventional kinesin that are involved in cargo association, folding and regulation, respectively. Using C-terminal and internal deletions, point mutations, localization studies, and an engineered ‘minimal’ kinesin, we identify five heptads of a coiled-coil domain in the kinesin tail that are necessary and sufficient for cargo association. Mutational analysis and in vitro ATPase assays highlight a conserved motif in the globular tail that is involved in regulation of the motor domain; a region preceding this motif participates in folding. Although these sites are spatially and functionally distinct, they probably cooperate during activation of the motor for cargo transport.

Cite this article

Seiler, S., Kirchner, J., Horn, C. et al. Cargo binding and regulatory sites in the tail of fungal conventional kinesin. Nat Cell Biol 2, 333–338 (2000). https://doi.org/10.1038/35014022

View full text

>> Full Text:   Cargo binding and regulatory sites in the tail of fungal conventional kinesin