Author: ["Dominique Pantaloni","Rajaa Boujemaa","Dominique Didry","Pierre Gounon","Marie-France Carlier"]
CITE.CC academic search helps you expand the influence of your papers.
Abstract
The Arp2/3 complex is an essential regulator of actin polymerization in response to signalling and generates a dendritic array of filaments in lamellipodia. Here we show that the activated Arp2/3 complex interacts with the barbed ends of filaments to initiate barbed-end branching. Barbed-end branching by Arp2/3 quantitatively accounts for polymerization kinetics and for the length correlation of the branches of filaments observed by electron microscopy. Filament branching is visualized at the surface of Listeria in a reconstituted motility assay. The functional antagonism between the Arp2/3 complex and capping proteins is essential in the maintenance of the steady state of actin assembly and actin-based motility.
Cite this article
Pantaloni, D., Boujemaa, R., Didry, D. et al. The Arp2/3 complex branches filament barbed ends: functional antagonism with capping proteins. Nat Cell Biol 2, 385–391 (2000). https://doi.org/10.1038/35017011