cAMP-GEFII is a direct target of cAMP in regulated exocytosis
Author: ["Nobuaki Ozaki","Tadao Shibasaki","Yasushige Kashima","Takashi Miki","Kazuo Takahashi","Hiroaki Ueno","Yasuhiro Sunaga","Hideki Yano","Yoshiharu Matsuura","Toshihiko Iwanaga","Yoshimi Takai","Susumu Seino"]
Publication: Nature Cell Biology
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Abstract
Although cAMP is well known to regulate exocytosis in many secretory cells, its direct target in the exocytotic machinery is not known. Here we show that cAMP-GEFII, a cAMP sensor, binds to Rim (Rab3-interacting molecule, Rab3 being a small G protein) and to a new isoform, Rim2, both of which are putative regulators of fusion of vesicles to the plasma membrane. We also show that cAMP-GEFII, through its interaction with Rim2, mediates cAMP-induced, Ca2+-dependent secretion that is not blocked by an inhibitor of cAMP-dependent protein kinase (PKA). Accordingly, cAMP-GEFII is a direct target of cAMP in regulated exocytosis and is responsible for cAMP-dependent, PKA-independent exocytosis.
Cite this article
Ozaki, N., Shibasaki, T., Kashima, Y. et al. cAMP-GEFII is a direct target of cAMP in regulated exocytosis. Nat Cell Biol 2, 805–811 (2000). https://doi.org/10.1038/35041046
