Author: ["Sushma Selvarajan","Leif R. Lund","Toshihiko Takeuchi","Charles S. Craik","Zena Werb"]
CITE.CC academic search helps you expand the influence of your papers.
Abstract
Here we show that plasma kallikrein (PKal) mediates a plasminogen (Plg) cascade in adipocyte differentiation. Ecotin, an inhibitor of serine proteases, inhibits cell-shape change, adipocyte-specific gene expression, and lipid accumulation during adipogenesis in culture. Deficiency of Plg, but not of urokinase or tissue-type plasminogen activator, suppresses adipogenesis during differentiation of 3T3-L1 cells and mammary-gland involution. PKal, which is inhibited by ecotin, is required for adipose conversion, Plg activation and 3T3-L1 differentiation. Human plasma lacking PKal does not support differentiation of 3T3-L1 cells. PKal is therefore a physiological regulator that acts in the Plg cascade during adipogenesis. We propose that the Plg cascade fosters adipocyte differentiation by degradation of the fibronectin-rich preadipocyte stromal matrix.Cite this article
Selvarajan, S., Lund, L., Takeuchi, T. et al. A plasma kallikrein-dependent plasminogen cascade required for adipocyte differentiation. Nat Cell Biol 3, 267–275 (2001). https://doi.org/10.1038/35060059 