Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia
Author: ["Mathias Köppen","Jeffrey S. Simske","Paul A. Sims","Bonnie L. Firestein","David H. Hall","Anthony D. Radice","Christopher Rongo","Jeffrey D. Hardin"]
Publication: Nature Cell Biology
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Abstract
The function of epithelial cell sheets depends on the integrity of specialized cell–cell junctions that connect neighbouring cells. We have characterized the novel coiled-coil protein AJM-1, which localizes to an apical junctional domain of Caenorhabditis elegans epithelia basal to the HMR–HMP (cadherin–catenin) complex. In the absence of AJM-1, the integrity of this domain is compromised. Proper AJM-1 localization requires LET-413 and DLG-1, homologues of the Drosophila tumour suppressors Scribble and Discs large, respectively. DLG-1 physically interacts with AJM-1 and is required for its normal apical distribution, and LET-413 mediates the rapid accumulation of both DLG-1 and AJM-1 in the apical domain. In the absence of both dlg-1 and let-413 function AJM-1 is almost completely lost from apical junctions in embryos, whereas HMP-1 (α-catenin) localization is only mildly affected. We conclude that LET-413 and DLG-1 cooperatively control AJM-1 localization and that AJM-1 controls the integrity of a distinct apical junctional domain in C. elegans.
Cite this article
Köppen, M., Simske, J., Sims, P. et al. Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia. Nat Cell Biol 3, 983–991 (2001). https://doi.org/10.1038/ncb1101-983
