Author: ["Gopal J. Babu","Evgenij Loukianov","Tatiana Loukianova","Gail J. Pyne","Sabine Huke","George Osol","Robert B. Low","Richard J. Paul","Muthu Periasamy"]
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Abstract
We used an exon-specific gene-targeting strategy to generate a mouse model deficient only in the SM-B myosin isoform. Here we show that deletion of exon-5B (specific for SM-B) in the gene for the heavy chain of smooth muscle myosin results in a complete loss of SM-B myosin and switching of splicing to the SM-A isoform, without affecting SM1 and SM2 myosin content. Loss of SM-B myosin does not affect survival or cause any overt smooth muscle pathology. Physiological analysis reveals that absence of SM-B myosin results in a significant decrease in maximal force generation and velocity of shortening in smooth muscle tissues. This is the first in vivo study to demonstrate a functional role for the SM-B myosin isoform. We conclude that the extra seven-residue insert in the surface loop 1 of SM-B myosin is a critical determinant of crossbridge cycling and velocity of shortening.Cite this article
Babu, G., Loukianov, E., Loukianova, T. et al. Loss of SM-B myosin affects muscle shortening velocity and maximal force development. Nat Cell Biol 3, 1025–1029 (2001). https://doi.org/10.1038/ncb1101-1025 