Myosin X is a downstream effector of PI(3)K during phagocytosis
Author: ["Dianne Cox","Jonathan S. Berg","Michael Cammer","John O. Chinegwundoh","Benjamin M. Dale","Richard E. Cheney","Steven Greenberg"]
Publication: Nature Cell Biology
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Abstract
Phagocytosis is a phosphatidylinositol-3-OH-kinase (PI(3)K)-dependent process in macrophages. We identified Myo10 (Myosin-X), an unconventional myosin with pleckstrin homology (PH) domains, as a potential downstream target of PI(3)K. Myo10 was recruited to phagocytic cups in a wortmannin-sensitive manner. Expression of a truncation construct of Myo10 (Myo10 tail) in a macrophage cell line or cytosolic loading of anti-Myo10 antibodies in bovine alveolar macrophages inhibited phagocytosis. In contrast, expression of a Myo10 tail construct containing a point mutation in one of its PH domains failed to inhibit phagocytosis. Expression of Myo10 tail inhibited spreading, but not adhesion, on IgG-coated substrates, consistent with a function for Myo10 in pseudopod extension. We propose that Myo10 provides a molecular link between PI(3)K and pseudopod extension during phagocytosis.
Cite this article
Cox, D., Berg, J., Cammer, M. et al. Myosin X is a downstream effector of PI(3)K during phagocytosis. Nat Cell Biol 4, 469–477 (2002). https://doi.org/10.1038/ncb805
