A kinase cascade leading to Rab11-FIP5 controls transcytosis of the polymeric immunoglobulin recepto
Author: ["Tao Su","David M. Bryant","Frédéric Luton","Marcel Vergés","Scott M. Ulrich","Kirk C. Hansen","Anirban Datta","Dennis J. Eastburn","Alma L. Burlingame","Kevan M. Shokat","Keith E. Mostov"]
Publication: Nature Cell Biology
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Abstract
Epithelial cell transcytosis of polymeric IgA by its receptor requires polarized membrane trafficking. A signalling cascade involving the tyrosine kinase YES, EGFR, ERK and the Rab11 effector FIP5 is now shown to regulate this process. Polymeric immunoglobulin A (pIgA) transcytosis, mediated by the polymeric immunoglobulin receptor (pIgR), is a central component of mucosal immunity and a model for regulation of polarized epithelial membrane traffic. Binding of pIgA to pIgR stimulates transcytosis in a process requiring Yes, a Src family tyrosine kinase (SFK). We show that Yes directly phosphorylates EGF receptor (EGFR) on liver endosomes. Injection of pIgA into rats induced EGFR phosphorylation. Similarly, in MDCK cells, pIgA treatment significantly increased phosphorylation of EGFR on various sites, subsequently activating extracellular signal-regulated protein kinase (ERK). Furthermore, we find that the Rab11 effector Rab11-FIP5 is a substrate of ERK. Knocking down Yes or Rab11-FIP5, or inhibition of the Yes–EGFR–ERK cascade, decreased pIgA–pIgR transcytosis. Finally, we demonstrate that Rab11-FIP5 phosphorylation by ERK controls Rab11a endosome distribution and pIgA–pIgR transcytosis. Our results reveal a novel Yes–EGFR–ERK–FIP5 signalling network for regulation of pIgA–pIgR transcytosis.
Cite this article
Su, T., Bryant, D., Luton, F. et al. A kinase cascade leading to Rab11-FIP5 controls transcytosis of the polymeric immunoglobulin receptor. Nat Cell Biol 12, 1143–1153 (2010). https://doi.org/10.1038/ncb2118
