Author: ["Marie Anne O’Donnell","Eva Perez-Jimenez","Andrew Oberst","Aylwin Ng","Ramin Massoumi","Ramnik Xavier","Douglas R. Green","Adrian T. Ting"]
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Abstract
Caspase 8 is known to suppress necroptosis, but its relevant target protein was unknown. Ting and colleagues show that caspase 8 cleaves the deubiquitylase CYLD to inhibit necroptosis and promote cell survival. Caspase 8 initiates apoptosis downstream of TNF death receptors by undergoing autocleavage and processing the executioner caspase 3 (ref. 1). However, the dominant function of caspase 8 is to transmit a pro-survival signal that suppresses programmed necrosis (or necroptosis) mediated by RIPK1 and RIPK3 (refs 2, 3, 4, 5, 6) during embryogenesis and haematopoiesis7,8,9. Suppression of necrotic cell death by caspase 8 requires its catalytic activity but not the autocleavage essential for apoptosis10; however, the key substrate processed by caspase 8 to block necrosis has been elusive. A key substrate must meet three criteria: it must be essential for programmed necrosis; it must be cleaved by caspase 8 in situations where caspase 8 is blocking necrosis; and mutation of the caspase 8 processing site on the substrate should convert a pro-survival response to necrotic death without the need for caspase 8 inhibition. We now identify CYLD as a substrate for caspase 8 that satisfies these criteria. Following TNF stimulation, caspase 8 cleaves CYLD to generate a survival signal. In contrast, loss of caspase 8 prevented CYLD degradation, resulting in necrotic death. A CYLD substitution mutation at Asp 215 that cannot be cleaved by caspase 8 switches cell survival to necrotic cell death in response to TNF.Cite this article
O’Donnell, M., Perez-Jimenez, E., Oberst, A. et al. Caspase 8 inhibits programmed necrosis by processing CYLD. Nat Cell Biol 13, 1437–1442 (2011). https://doi.org/10.1038/ncb2362 