Fibroblast polarization is a matrix-rigidity-dependent process controlled by focal adhesion mechanos
Author: ["Masha Prager-Khoutorsky","Alexandra Lichtenstein","Ramaswamy Krishnan","Kavitha Rajendran","Avi Mayo","Zvi Kam","Benjamin Geiger","Alexander D. Bershadsky"]
Publication: Nature Cell Biology
CITE.CC academic search helps you expand the influence of your papers.
Abstract
Cell elongation and polarization are basic morphogenetic responses to extracellular matrix adhesion. We demonstrate here that human cultured fibroblasts readily polarize when plated on rigid, but not on compliant, substrates. On rigid surfaces, large and uniformly oriented focal adhesions are formed, whereas cells plated on compliant substrates form numerous small and radially oriented adhesions. Live-cell monitoring showed that focal adhesion alignment precedes the overall elongation of the cell, indicating that focal adhesion orientation may direct cell polarization. siRNA-mediated knockdown of 85 human protein tyrosine kinases (PTKs) induced distinct alterations in the cell polarization response, as well as diverse changes in cell traction force generation and focal adhesion formation. Remarkably, changes in rigidity-dependent traction force development, or focal adhesion mechanosensing, were consistently accompanied by abnormalities in the cell polarization response. We propose that the different stages of cell polarization are regulated by multiple, PTK-dependent molecular checkpoints that jointly control cell contractility and focal-adhesion-mediated mechanosensing. Bershadsky and colleagues show that fibroblast polarization depends on matrix rigidity and focal adhesion mechanosensing. They target protein tyrosine kinases through RNAi to identify signalling molecules that regulate traction force generation, focal adhesion assembly and mechanosensitivity.
Cite this article
Prager-Khoutorsky, M., Lichtenstein, A., Krishnan, R. et al. Fibroblast polarization is a matrix-rigidity-dependent process controlled by focal adhesion mechanosensing. Nat Cell Biol 13, 1457–1465 (2011). https://doi.org/10.1038/ncb2370