Author: ["Noriaki Shimizu","Kotaro Sugimoto","Jianwei Tang","Takeyuki Nishi","Iwao Sato","Masaki Hiramoto","Shin Aizawa","Mamoru Hatakeyama","Reiko Ohba","Hideaki Hatori","Tatsufumi Yoshikawa","Fumihiko Suzuki","Akira Oomori","Hirotoshi Tanaka","Haruma Kawaguchi"
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Abstract
We have developed a method using novel latex beads for rapid identification of drug receptors using affinity purification. Composed of a glycidylmethacrylate (GMA) and styrene copolymer core with a GMA polymer surface, the beads minimize nonspecific protein binding and maximize purification efficiency. We demonstrated their performance by efficiently purifying FK506-binding protein using FK506-conjugated beads, and found that the amount of material needed was significantly reduced compared with previous methods. Using the latex beads, we identified a redox-related factor, Ref-1, as a target protein of an anti-NF-κB drug, E3330, demonstrating the existence of a new class of receptors of anti-NF-κB drugs. Our results suggest that the latex beads could provide a tool for the identification and analysis of drug receptors and should therefore be useful in drug development.Cite this article
Shimizu, N., Sugimoto, K., Tang, J. et al. High-performance affinity beads for identifying drug receptors. Nat Biotechnol 18, 877–881 (2000). https://doi.org/10.1038/78496 