Author: ["Georgette E. Cole","Peter C. McCabe","Duane Inlow","David H. Gelfand","Arie Ben-Bassat","Michael A. Innis"]
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Abstract
A strain of distiller's yeast stably secreting high levels of Aspergillus awamori glucoamylase was constructed by introducing an expression cassette containing glucoamylase cDNA and a dominant selectable marker into the yeast chromosome. Glucoamylase expression levels were increased by modifying the yeast promoter and the sequences upstream of the start of translation. Fermentation studies with the improved strain showed that production of glucoamylase was constant for over 800 generations of continuous culture. This strain utilizes about 95% of the carbohydrates in soluble starch at 25% (w/v) substrate concentration and produces high yields of ethanol.Cite this article
Cole, G., McCabe, P., Inlow, D. et al. Stable Expression of Aspercillus Awamori Glucoamylase in Distiller's Yeast. Nat Biotechnol 6, 417–421 (1988). https://doi.org/10.1038/nbt0488-417 