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Abstract
Cells of the organism Alcaligenes denitrificans were immobilised in Celite, particles of calcined diatomaceous earths. The kinetics of adsorption of non-growing cells was tested under varying conditions of cell concentration, salt concentration and pre-saturation of the beads with growth substrate. The adsorption of cells was also compared against other common biomass carrier particles such as sand, charcoal and anthracite. From this, a method for the immobilisation of cells onto Celite was developed, and cells immobilised by this method were used in a bubble column reactor, operated continuously at a dilution rate greater than the apparent maximum exponential growth rate of the free cells. The physical presence of cells on the diatomaceous earth particles was studied using scanning electron microscopy.
Cite this article
Caunt, P., Chase, H. Biodegradation by Bacteria Immobilised on Celite Particles. Nat Biotechnol 6, 721–725 (1988). https://doi.org/10.1038/nbt0688-721