Human B-Cell Stimulatory Factor-2 Expressed in Escherichia Coli
Author: ["Yoshihiro Asagoe","Kiyoshi Yasukawa","Takashi Saito","Naoko Maruo","Kenji Miyata","Toshiaki Kono","Toshio Miyake","Takao Kato","Hitoshi Kakidani","Mitsuaki Mitani"]
Publication: Bio/Technology
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Abstract
Human B-cell stimulatory factor-2 (BSF-2/ IL-6) is an important cytokine regulating the growth and differentiation of cells as diverse as lymphocytes, hepatocytes, and neurons. Recently its aberrant expression has been correlated with several diseases. Starting with a previously isolated cDNA clone encoding human BSF-2, we constructed two types of plasmids from which peptides containing BSF-2 were produced in Escherichia coli as human growth hormone (hGH) fusion proteins. The products were solubilized, and cleaved by factor Xa, a specific endopeptidase, to liberate Glu-Phe-Met-BSF-2 or Ala-BSF-2, which were purified to homogeneity. The recombinant products were indistinguishable from natural BSF-2 as assessed by their B-cell stimulatory activities.
Cite this article
Asagoe, Y., Yasukawa, K., Saito, T. et al. Human B-Cell Stimulatory Factor-2 Expressed in Escherichia Coli. Nat Biotechnol 6, 806–809 (1988). https://doi.org/10.1038/nbt0788-806
