Genetic Construction of Lactose-Utilizing Strains of Pseudomonas Aeruginosa and Their Application in

Abstract

We inserted the E. coli lacZY genes into the chromosomes of Pseudomonas aeruginosa strains PAO-1 and PG-201 using a bicomponent transposition system, and obtained transconjugant strains with one set of lacZY genes integrated into the chromosomes at unique locations. The strains were positive in the o-nitrophenylgalacto-side (ONPG) test and produced E. coli β-galactosidase-related proteins in Western blots. The transconjugants grew well in lactose-based media (minimal medium and whey) albeit with reduced initial rates as compared to growth in glucose-based minimal media. Pseudomonas rhamnolipids were produced during stationary growth in lactose-based minimal media and whey, showing that waste products can be effectively used for important bio-technological processes.

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Koch, A., Reiser, J., Käppeli, O. et al. Genetic Construction of Lactose-Utilizing Strains of Pseudomonas Aeruginosa and Their Application in Biosurfactant Production. Nat Biotechnol 6, 1335–1339 (1988). https://doi.org/10.1038/nbt1188-1335

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