Author: ["Edgar Ong","Neil R. Gilkes","R. Antony J. Warren","Robert C. Miller Jr.","Douglas G. Kilburn"]
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Abstract
We have constructed a fusion between the gene (abg) for a β-glucosidase (Abg) of an Agrobacterium sp. and part of the gene (cex) for an exoglucanase (Cex) of Cellulomonas fimi. The abg-cex fusion encodes a fusion protein (Abg-CBDCex) with the cellulose-binding properties of Cex and β-glucosidase activity of Abg. The fusion protein retained 42 percent of its β-glucosidase activity when bound to cellulose. The binding was stable enough to allow continuous hydrolysis of substrate by the enzyme without detectable leaching of the enzyme from the cellulose. We believe that the cellulose-binding domain (CBDCex) of Cex offers a simple, inexpensive and widely applicable method of enzyme immobilization.Cite this article
Ong, E., Gilkes, N., Warren, R. et al. Enzyme Immobilization Using the Cellulose-Binding Domain of a Cellulomonas Fimi Exoglucanase. Nat Biotechnol 7, 604–607 (1989). https://doi.org/10.1038/nbt0689-604 