Author: ["Ken-ichiro Miyagawa","Naoyuki Kanzaki","Hiroyuki Kimura","Yasuhiro Sumino","Shun-ichi Akiyama","Yoshio Nakao"]
CITE.CC academic search helps you expand the influence of your papers.
Abstract
We have devised a direct selection for auxotrophic mutant isolation, and report here its use to obtain a xanthine-requiring mutant of B. subtilis that shows significantly improved inosine production. Insertion of a chloramphenicol acetyl transferase gene into a cloned B. subtilis IMP dehydrogenase gene on pBR322 was followed by transformation of inosine-guanosine producing B. subtilis with a fragment of the mutagenized plasmid. All chloramphenicol resistant transformants were xanthine dependent and accumulated almost twice as much inosine as the parental strain.Cite this article
Miyagawa, Ki., Kanzaki, N., Kimura, H. et al. Increased Inosine Production by a Bacillus subtilis Xanthine-Requiring Mutant Derived by Insertional Inactivation of the IMP Dehydrogenase Gene. Nat Biotechnol 7, 821–824 (1989). https://doi.org/10.1038/nbt0889-821 