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Abstract
We report plantlet regeneration from conifer protoplasts. White spruce protoplasts were isolated from previously cryopreserved embryogenic suspension cultures and cultured in PCM with an osmoticum containing myo-inositol. Protoplast derived embryos established in suspension culture, were used as source material for plantlet regeneration. Embryo maturation was induced on 1/2 strength LP medium containing 90 mM sucrose and 12 μM abscisic acid. After 4 weeks, cultures were transferred to 1/2 strength LP medium, 60 mM sucrose and no growth regulators. Approximately 7–14 days after transfer to this medium, embryos bearing prominent cotyledons were separated from the main callus and cultured independently. These embryos underwent cotyledon and hypo-cotyl elongation and root development. The total time to plantlet recovery, following protoplast isolation was 15 weeks.Cite this article
Attree, S., Dunstan, D. & Fowke, L. Plantlet Regeneration from Embryogenic Protoplasts of White Spruce (Picea Glauca). Nat Biotechnol 7, 1060–1062 (1989). https://doi.org/10.1038/nbt1089-1060 