Author: ["Jian Lv","Kun Yu","Juan Wei","Huaping Gui","Chunxia Liu","Dawei Liang","Yanli Wang","Hongju Zhou","Ryan Carlin","Randy Rich","Tiancong Lu","Qiudeng Que","Wen Chung Wang","Xingping Zhang","Tim Kelliher"]
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Abstract
New breeding technologies accelerate germplasm improvement and reduce the cost of goods in seed production1–3. Many such technologies could use in vivo paternal haploid induction (HI), which occurs when double fertilization precedes maternal (egg cell) genome loss. Engineering of the essential CENTROMERIC HISTONE (CENH3) gene induces paternal HI in Arabidopsis4–6. Despite conservation of CENH3 function across crops, CENH3-based HI has not been successful outside of the Arabidopsis model system7. Here we report a commercially operable paternal HI line in wheat with a ~7% HI rate, identified by screening genome-edited TaCENH3α-heteroallelic combinations. Unlike in Arabidopsis, edited alleles exhibited reduced transmission in female gametophytes, and heterozygous genotypes triggered higher HI rates than homozygous combinations. These developments might pave the way for the deployment of CENH3 HI technology in diverse crops. Gene editing induces paternal haploid plants in hexaploid wheat.Cite this article
Lv, J., Yu, K., Wei, J. et al. Generation of paternal haploids in wheat by genome editing of the centromeric histone CENH3. Nat Biotechnol 38, 1397–1401 (2020). https://doi.org/10.1038/s41587-020-0728-4 