Probing the biophysical interplay between a viral genome and its capsid
Author: ["J. Snijder","C. Uetrecht","R. J. Rose","R. Sanchez-Eugenia","G. A. Marti","J. Agirre","D. M. A. Guérin","G. J. L. Wuite","A. J. R. Heck","W. H. Roos"]
Publication: Nature Chemistry
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Abstract
The interaction between a viral capsid and its genome governs crucial steps in the life cycle of a virus, such as assembly and genome uncoating. Tuning cargo–capsid interactions is also essential for successful design and cargo delivery in engineered viral systems. Here we investigate the interplay between cargo and capsid for the picorna-like Triatoma virus using a combined native mass spectrometry and atomic force microscopy approach. We propose a topology and assembly model in which heterotrimeric pentons that consist of five copies of structural proteins VP1, VP2 and VP3 are the free principal units of assembly. The interpenton contacts are established primarily by VP2. The dual role of the genome is first to stabilize the densely packed virion and, second, on an increase in pH to trigger uncoating by relaxing the stabilizing interactions with the capsid. Uncoating occurs through a labile intermediate state of the virion that reversibly disassembles into pentons with the concomitant release of protein VP4. The stability of the capsid of a virus is strongly affected by its genome. Here the interplay between capsid and genome is explored using native mass spectrometry and atomic force microscopy. A mechanism is proposed to explain how the genome of the Triatoma virus stabilizes the capsid at neutral pH, but triggers disassembly under alkaline conditions.
Cite this article
Snijder, J., Uetrecht, C., Rose, R. et al. Probing the biophysical interplay between a viral genome and its capsid. Nature Chem 5, 502–509 (2013). https://doi.org/10.1038/nchem.1627
