Centrosome duplication in mammalian somatic cells requires E2F and Cdk2–Cyclin A

Abstract

Centrosome duplication is a key requirement for bipolar spindle formation and correct segregation of chromosomes during cell division. In a manner highly reminiscent of DNA replication, the centrosome must be duplicated once, and only once, in each cell cycle. How centrosome duplication is regulated and coordinated with other cell-cycle functions remains poorly understood. Here, we have established a centrosome duplication assay using mammalian somatic cells. We show that centrosome duplication requires the activation of E2F transcription factors and Cdk2–cyclin A activity.

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Meraldi, P., Lukas, J., Fry, A. et al. Centrosome duplication in mammalian somatic cells requires E2F and Cdk2–Cyclin A. Nat Cell Biol 1, 88–93 (1999). https://doi.org/10.1038/10054

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