β-Arrestins regulate a Ral-GDS–Ral effector pathway that mediates cytoskeletal reorganization
Author: ["Moshmi Bhattacharya","Pieter H. Anborgh","Andy V. Babwah","Lianne B. Dale","Tomas Dobransky","Jeffery L. Benovic","Ross D. Feldman","Joseph M. Verdi","R. Jane Rylett","Stephen S. G. Ferguson"]
Publication: Nature Cell Biology
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Abstract
β-Arrestins are important in chemoattractant receptor-induced granule release, a process that may involve Ral-dependent regulation of the actin cytoskeleton. We have identified the Ral GDP dissociation stimulator (Ral-GDS) as a β-arrestin-binding protein by yeast two-hybrid screening and co-immunoprecipitation from human polymorphonuclear neutrophilic leukocytes (PMNs). Under basal conditions, Ral-GDS is localized to the cytosol and remains inactive in a complex formed with β-arrestins. In response to formyl-Met-Leu-Phe (fMLP) receptor stimulation, β-arrestin–Ral-GDS protein complexes dissociate and Ral-GDS translocates with β-arrestin from the cytosol to the plasma membrane, resulting in the Ras-independent activation of the Ral effector pathway required for cytoskeletal rearrangement. The subsequent re-association of β-arrestin–Ral-GDS complexes is associated with the inactivation of Ral signalling. Thus, β-arrestins regulate multiple steps in the Ral-dependent processes that result in chemoattractant-induced cytoskeletal reorganization.
Cite this article
Bhattacharya, M., Anborgh, P., Babwah, A. et al. β-Arrestins regulate a Ral-GDS–Ral effector pathway that mediates cytoskeletal reorganization. Nat Cell Biol 4, 547–555 (2002). https://doi.org/10.1038/ncb821