How APC/C–Cdc20 changes its substrate specificity in mitosis

Abstract

APC/C-Cdc20 ubiquitylates different substrates at distinct times during the cell cycle. This substrate specificity is now shown to depend on the differential binding of Cdc20 to APC subunits in response to the state of activation of the spindle assembly checkpoint. Progress through mitosis requires that the right protein be degraded at the right time. One ubiquitin ligase, the anaphase-promoting complex or cyclosome (APC/C) targets most of the crucial mitotic regulators by changing its substrate specificity throughout mitosis. The spindle assembly checkpoint (SAC) acts on the APC/C co-activator, Cdc20 (cell division cycle 20), to block the degradation of metaphase substrates (for example, cyclin B1 and securin), but not others (for example, cyclin A). How this is achieved is unclear. Here we show that Cdc20 binds to different sites on the APC/C depending on the SAC. Cdc20 requires APC3 and APC8 to bind and activate the APC/C when the SAC is satisfied, but requires only APC8 to bind the APC/C when the SAC is active. Moreover, APC10 is crucial for the destruction of cyclin B1 and securin, but not cyclin A. We conclude that the SAC causes Cdc20 to bind to different sites on the APC/C and this alters APC/C substrate specificity.

Cite this article

Izawa, D., Pines, J. How APC/C–Cdc20 changes its substrate specificity in mitosis. Nat Cell Biol 13, 223–233 (2011). https://doi.org/10.1038/ncb2165

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